The development and spread of bacterial strains that are resistant to antibacterial drugs has emerged as a global problem. aureus ATCC 25923, an all-sensitive reference strain, was used as a quality control strain for the DAD test. This gave a profile of drug susceptibility vis-à-vis antibiotic resistance. The diameter of zone of bacterial growth inhibition surrounding the disc (including the disc) was measured and compared with the standard for each drug. Antibiotic discs were obtained commercially from Himedia. Filter paper discs of 6 mm size were used to observe antibiotic susceptibility patterns against 11 antibiotics. With this culture, a bacterial lawn was prepared on Mueller-Hinton agar. The tested bacterium was from an overnight culture (inoculated from a single colony) and freshly grown for 4 hours at approximately 10 6 CFU/mL. Susceptibility of isolates to imipenem, amikacin, kanamycin and oxacillin, were determined by DAD technique according to Acar and Bauer et al. aureus strains, and antibiotic susceptibility against some conventional and traditional antibiotics. The present study aimed to observe the biochemical characters of isolated S. Out of them twenty two were vancomycin-sensitive and the rests were vancomycin-resistant. aureus from post operative pus sample by standard biochemical test and detected specific nuc gene of S. Recently, we have isolated thirty pathogenic S. In India, it is reported that 70% of the strains are resistant to methicillin in some cities. High prevalence of MRSA in hospitals has been reported from many states of India. aureus (MRSA) is mediated by the presence of PBP-2a which is expressed by an exogenous gene, mecA. aureus but resistance finally emerged in 1962. Methicillin, a semi synthetic penicillin was used to treat penicillin resistant S. aureus strains are resistant to penicillin. In 1944, by destroying the penicillin by penicillinase, S. Penicillin was the first choice of antibiotics to treat staphylococcal infection. aureus has developed resistance to most classes of antimicrobial agents. aureus causes superficial skin infections and life-threatening diseases such as endocarditis, sepsis and soft tissue, urinary tract, respiratory tract, intestinal tract, bloodstream infections. aureus), a gram positive cocci, is a major human pathogen causing large variety of infections worldwide and predominates in surgical wound infections with prevalence rate ranging from 4.6%-54.4% –.
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